Chondroitin Sulfate Mouse anti-Human, Alexa Fluor 647, Clone: 9.2.27, BD

Description

• The 9.2.27 monoclonal antibody specifically binds to CSPG4 (chondroitin sulfate proteoglycan 4)
• CSPG4 is also known as MCSP (melanoma chondroitin sulfate proteoglycan) and NG2 (neural/glial antigen 2)
• The hybridoma secreting the 9.2.27 antibody was generated using a human melanoma cell extract as the immunogen
• Tumor cells display a variety of antigens which have been defined by antibodies
• Antibodies that specifically react with tumor-associated antigens are useful in understanding the biology of particular tumor types
• Proteoglycans including chondroitin sulfate, keratan sulfate, dermatan sulfate and heparan sulfate are major components of the extracellular matrices of many animal cell types
• Clone 9.2.27 has been shown to react with human melanoma cells, glioma cells, and proliferating brain endothelial cells
• It did not react with fetal melanocytes, neuroblastoma LA-N-1 cells or a variety of carcinoma, lymphoid, and fibroblastoid cell lines
• Additionally, neither white nor gray matter from the medulla oblongata, cerebellum or spinal cord of a normal human adult reacted with 9.2.27
• Clone 9.2.27 has also been used in functional studies to investigate the role of chondroitin sulfate proteoglycans in human tumor cell systems
• It has been shown to block melanoma cell spreading, exhibit antiproliferative effects on glioma cells in vitro and suppress glioma tumor growth in athymic nude mice models.