FoxP3 Mouse anti-Human, PE-CF594, Clone: 259D/C7, BD

Description

• The 259D/C7 antibody monoclonal antibody specifically binds to the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors
• The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells
• Flow cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive
• Approximately 5-10% of peripheral CD4+ cells are CD4+CD25+ T regulatory cells
• T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells
• To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice
• The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488nm), green (532nm) and yellow-green (561nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20nm filter)
• Host Species: Mouse Clone: 259D/C7 Isotype: IgG1 Species Reactivity: Human Immunogen: FoxP3 recombinant protein Intracellular Staining