Smad1/Smad8 Rat, PE, Clone: N6-1233, BD

Description

• The N6-1233 monoclonal antibody specifically binds to the Smad1 protein phosphorylated at the Ser463/465 sites and the Smad8 protein phosphorylated at the Ser465/467 sites
• Smad1 and Smad8 are ∽60 kDa proteins and are members of the Smad superfamily
• The Smad family members are divided into three subfamilies: receptor regulated Smads or R-Smads, including Smads1, 2, 3, 5, and 8; common partner Smad, or Co-Smad, including Smad4; and inhibitory Smads, or I-Smad, including Smads 6 and 7
• Activation of Transforming Growth Factor-beta (TGF-beta) superfamily serine/threonine kinase receptors, such as TGF-beta and Bone MorphogenicProtein (BMP) receptors, leads to the phosphorylation of R-Smads at several sites
• It has been shown that Ser463 and Ser465 of Smad1 are phosphorylated by BMP receptors
• In B cells and pre-B cells, BMP-6 has been shown to induce Smad1/5/8 phosphorylation and inhibit cell proliferation
• Phosphorylated R-Smads form complexes with Co-Smad and translocate into the nucleus to regulate transcription affecting a wide range of critical processes including cell-fate determination, proliferation, morphogenesis, differentiation and apoptosis
• The inhibitory Smads inhibit this pathway through two potential mechanisms: either by preventing R-Smads from binding to their corresponding receptors and/or by competing with Smad4, the Co-Smad, from binding to R-Smads
• This antibody may crossreact with Smad5 pS463/pS465 based on sequence homology.