CD38 Mouse anti-Human, Brilliant Violet 605, Clone: HB7, BD

Description

• The HB7 monoclonal antibody specifically binds to human CD38
• CD38 is a type II transmembrane glycoprotein of 45 kDa with a protein core of 35 kDa
• The CD38 antigen is expressed on essentially all pre-B lymphocytes, plasma cells, and thymocytes
• It is also present on activated T lymphocytes, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts
• The antigen is expressed during the early stages of T- and B-lymphocyte differentiation, is lost during the intermediate stages of maturation, and then reappears during the final stages of maturation
• The CD38 antigen is expressed on 90% of CD34+ cells, and is not expressed on pluripotent stem cells
• Coexpression of CD38 antigen on CD34+ cells indicates lineage commitment of those cells
• CD38 is a counter-receptor of CD31
• It is also expressed in T- and B-acute lymphoblastic leukemia (ALL), Burkitt's lymphoma, multiple myeloma, acute myeloid leukemia (AML), and chronic lymphocytic leukemia (CLL)
• This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.