Siglec-F Rat anti-Mouse, Brilliant Violet 421, Clone: E50-2440, BD

Description

• The E50-2440 antibody reacts with Siglec-F, the sixth siglec protein to be reported in the mouse
• Siglecs are the s ialic acid-binding immunoglobulin superfamily lectins defined in the human, each of which has a distinctive expression pattern in the hematopoietic system and at least some of which are known to mediate cell-cell interactions
• Orthologous proteins of human Siglec-1 (Sialoadhesin or CD169), Siglec-2 (CD22), and Siglec-4 (myelin-associated glycoprotein) have been characterized in the mouse
• Human Siglec-3 (CD33) and Siglecs-5 through -10 are encoded by a cluster of closely related genes, and each has two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motifs)
• Similarly, mouse Siglec-F is encoded by a gene in a syntenic cluster in the mouse, and the protein has sialic acid-binding activity and an intracytoplasmic ITIM
• Its expression pattern differs from those of the human Siglec-3-related proteins in that it is found on immature cells of the myelomonocytic lineage, with reduced expression on mature neutrophils and monocytes, and not on lymphoid cells
• It has been proposed that mAb E50-2440 may be used for identification of immature myelomonocytic cells in the mouse.