IL-8 Mouse anti-Human, Brilliant Violet 421, Clone: G265-8, BD

Description

• The G265-8 monoclonal antibody specifically binds to both the 72 and 77 amino acid isoforms of human Interleukin-8 (IL-8)
• IL-8 is secreted as an 8-9 kDa, non-glycosylated proinflammatory chemokine protein also known as chemokine (C-X-C motif) ligand 8 (CXCL8)
• IL-8 is synthesized as a 99 amino acid precursor that is proteolytically processed into several isoforms
• The 72 amino acid isoform is produced by monocytes, macrophages, granulocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli including cytokines and microbial agents
• It is also expressed by endothelial cells, fibroblasts, keratinocytes, lymphocytes, and a variety of tumor cells
• In response to IL-4, IL-10 and TGFβ, the cellular production of IL-8 is inhibited
• IL-8 is crucial for the activation and recruitment of neutrophils to inflammatory sites
• IL-8 is also a chemoattractant for basophils and T-lymphocytes
• IL-8 possesses angiogenic activity and can be associated with tumor angiogenesis and metastasis
• The 77 amino acid IL-8 isoform is primarily produced by endothelial cells
• This larger isoform is reportedly a less potent neutrophil activator than the 72 amino acid isoform
• IL-8 binds to and signals through two G-protein-coupled receptors, IL-8RA (CXCR1/CD181) and IL-8RB (CXCR2/CD182)
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.