CD49F Rat anti-Human, Brilliant Violet 650, Clone: GOH3, BD

Description

• The GoH3 monoclonal antibody specifically binds to CD49f which is also known as i ntegrin α6 chain
• CD49f is a ∽150 kDa type I transmembrane glycoprotein that belongs to the integrin alpha chain family of extracellular matrix and cell adhesion receptors
• The i ntegrin α6 subunit associates with the i ntegrin β1 chain (CD29) to form VLA-6 and with the integrin β4 chain (CD104) to form the integrin α6β4 complex, also known as the laminin and kalinin receptor
• CD49f is ex pressed mainly on T cells, monocytes, platelets, epithelial cells, endothelial cells, perineural cells, and trophoblasts of placenta
• GoH3 recognizes an extracellular epitope of integrin α6 on human, mouse and bovine cells
• GoH3 has been reported to block the binding of integrin α6 to laminin P1 and E8 fragments
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.