CD62L Mouse anti-Human, Brilliant Violet 650, Clone: DREG-56, BD

Description

• The DREG-56 monoclonal antibody specifically binds to CD62L
• CD62L is a 76-95 kDa glycoprotein that is also referred to as L-selectin or LECAM-1
• CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells
• The DREG-56 antibody recognizes the same antigen as LAM-1, and specifically inhibits >90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue
• It thus defines L-selectin as a human lymphocyte homing receptor for peripheral lymph node HEV
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.