CD69 Mouse anti-Human, Brilliant Violet 650, Clone: FN50, BD

Description

• The FN50 monoclonal antibody specifically binds to human CD69
• CD69 is also known as activation-induced molecule (AIM), early activation antigen (EA-1), very early activation antigen (VEA), C-type lectin domain family 2 member C (CLEC2C), MLR-3, GP32/28 and Leu-23
• CD69 is a transmembrane type II homodimer receptor
• CD69 is comprised of disulfide-linked, differentially glycosylated core protein subunits that are approximately 28 and 34 kDa in size
• Each subunit contains a C-type lectin domain
• CD69 is expressed on activated T, B, and natural killer (NK) lymphocytes, thymocytes, neutrophils, eosinophils and platelets
• In normal peripheral blood, a small and variable percentage of lymphocytes typically express detectable membrane CD69 antigen
• Upon activation, CD69 antigen expression increases on lymphocytes
• Peak CD69 expression generally occurs within 18 hours of activation, preceding the appearance of HLA-DR, IL-2Rα (CD25) and transferrin receptor (CD71)
• CD69 is highly expressed on the bright CD3+ subset of thymocytes
• FN50 monoclonal antibody labels NK cells and most lymphocytes of the follicular mantle and perifollicular/interfollicular zone as well as germinal center T cells of lymph nodes and tonsils
• Studies indicate that CD69 serves as a signaling receptor in the activation of a variety of cell types
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.