CD163 Mouse anti-Human, Brilliant Violet 650, Clone: GHI/61, BD

Description

• The GHI/61 monoclonal antibody specifically binds to human CD163
• CD163 is also known as Scavenger receptor cysteine-rich type 1 protein M130 (M130), Hemoglobin scavenger receptor and Macrophage-associated antigen
• CD163 is a 110-130 kDa transmembrane glycoprotein
• CD163 is a monocyte/macrophage-restricted antigen expressed on the majority of tissue macrophages and peripheral blood monocytes
• CD163 belongs to the scavenger receptor superfamily
• Its expression on monocytes is upregulated upon cellular activation
• CD163 expression reportedly changes on monocytes and macrophages as these cells differentiate
• This finding suggests a role for this molecule in the differentiation and/or regulation of monocyte and macrophage function
• CD163 may play a role in the clearance and endocytosis of hemoglobin and haptoglobin complexes by macrophages
• It has been reported (Maniecki et al., 2011) that the presence of calcium impacts the binding affinity of clone GHI/61 to CD163
• There is a variation in detecting CD163 positive monocytes when the cells are prepared with different anticoagulants, where heparin was observed to have the highest inhibitory effect on clone GHI/61
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.