IL-13 Rat anti-Human, Brilliant Violet 711, Clone: JES10-5A2, BD

Description

• The JES10-5A2 monoclonal antibody specifically binds to human interleukin-13, IL-13
• IL-13 is produced by activated T cells, mast cells and NK cells
• IL-13 regulates IgE production by B cells and can suppress the cytotoxic activity of macrophages and their production of inflammatory mediators
• The immunogen used to produce the JES10-5A2 hybridoma was COS-expressed recombinant human IL-13
• This is a neutralizing antibody
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.