CD107a Rat anti-Mouse, Brilliant Violet 711, Clone: 1D4B, BD

Description

• The 1D4B antibody recognizes CD107a (Lysosome-Associated Membrane Protein 1, LAMP-1), one of the two major glycoproteins in lysosome membranes which are useful markers to distinguish lysosomes from other organelles
• Mouse CD107a consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa
• It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice
• Several lines of evidence suggest that cell-surface mouse and human LAMP-1 glycoproteins, the expression of which may correlate with malignant transformation, participate in intercellular adhesion and adhesion to extracellular matrix
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.