CD25 Rat anti-Mouse, BB515, Clone: PC61, BD

Description

• The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested
• IL-2Rα by itself is not a signaling receptor
• However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2
• Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα
• CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells
• It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus
• Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance
• It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat
• No
• 553068)
• The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat
• No
• 557364) and 7D4 (Cat
• No
• 553068)
• It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25
• The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.