CD3 Mouse anti-Human, BB515, Clone: UCHT1, BD

Description

• The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
• CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
• Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
• CD3 plays a central role in signal transduction during antigen recognition
• The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
• The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.