Btk (pY223)/Itk (pY180) Mouse, Brilliant Violet 421, Clone: N35-86, BD

Description

• The N35-86 reacts with human and mouse Bruton's tyrosine kinase that is phosphorylated at the tyrosine 223 position, Btk (pY223)
• Btk is also known as agammaglobulinaemia tyrosine kinase (ATK) and B-cell progenitor kinase (BPK)
• Btk is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling
• It is a member of the Tec family of kinases which includes Tec and Itk
• In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain
• The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551
• This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain
• Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate
• The PH domain is essential for proper activation and function of Btk
• A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia
• The orthologous phosphorylation site for rat BTK is Y224
• Crossreactivity with human Itk (pY180) was confirmed by immunoprecipitation and Western blot analyses using the N35-86 antibody
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.