Ig Goat anti-Mouse, BB515, Polyclonal, BD

Description

• BD Horizon™ BB515 Goat Anti-Mouse Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Mouse Ig primary antibodies
• It is reactive with the heavy and light chains of mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes
• Minimal background staining of human and rat cells occurs in the absence of a primary mouse antibody
• In addition, the BD Horizon BB515 Goat Anti-Mouse Ig stains mouse B lymphocytes with little non-specific staining of other cells
• Therefore, it is useful as a primary immunofluorescent reagent for staining mouse B cells and antibody-producing cells and as a secondary reagent for staining human and rat cells pre-stained with mouse Ig primary antibodies
• The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.