CD273 Rat anti-Mouse, BUV395, Clone: TY25, BD

Description

• The TY25 monoclonal antibody specifically binds to CD273, also known as B7-DC or PD-L2
• CD273 is a 42-kDa type I membrane glycoprotein encoded by the Pdcd1lg2 gene of the B7 family of the Ig superfamily
• Pdcd1lg2 mRNA is expressed selectively in dendritic cells (DC), liver, and a few transformed cell lines (hepatic, neuroblastoma, and myeloid), but not in lymphoid tissues or macrophages
• The protein has not been detected on resting peripheral T and B lymphocytes, macrophages, or DC, but it is upregulated upon activation of T cells, macrophages, and DC by a variety of stimulatory factors
• B7-DC's receptor, PD-1, contains an ( I mmunoreceptor T yrosine-based I nhibitory M otif) on its intracytoplasmic region and is expressed on activated B and T lymphocytes, suggesting that B7-DC-PD-1 interaction may be involved in the negative regulation of immune responses
• The second PD-1 ligand, B7-H1 (PD-L1), is also a member of the B7 family of the Ig superfamily
• B7-DC may also participate in positive immunoregulation, or costimulation of T cells, through an additional receptor, which is not PD-1 and distinct from the alternate receptor for B7-H1
• Moreover, B7-DC is the first B7 ligand that has been found to participate in bi-directional communication; ligation of B7-DC on DC stimulates those DC
• The TY25 antibody blocks the binding of B7-DC to PD-1, but not to its alternate ligand
• The antibody was conjugated to BD Horizon BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
• With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector
• BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.