CD8 Mouse anti-Human, APC-R700, Clone: RPA-T8, BD

Description

• The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α)
• CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily
• CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells
• Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers
• CD8 plays important roles in T cell activation and selection
• The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen
• The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling
• The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking
• This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey
• In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors
• This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700
• APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness
• This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).