NF-κB p65 Mouse anti-Human, Brilliant Violet 421, Clone: K10-895.12.50, BD

Description

• The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of the human NF- κ B p65 subunit
• Nuclear factor κ B (NF- κ B) is a ubiquitously expressed transcription factor that regulates the expression of many other genes
• It is crucial for cellular responses to a variety of stimuli including stress and microbial pathogens that lead to immunity, inflammation, proliferation, differentiation, survival, apoptosis, and tumorigenesis
• The most studied NF- κ B complex consists of the p50 (also known as NF- κ B1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain)
• The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences
• In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor I κ B and which contains phosphorylation sites
• In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I κ κ B/NF- κ B complex, allowing the free heterodimeric NF- κ B to translocate to the nucleus
• Furthermore, optimal activation of NF- κ B requires phosphorylation in the transactivation domain of p65
• In the nucleus, activated NF- κ B dimers bind to the κ B sites within promoters and enhancers and function as transcriptional regulators
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.