BV480 CD138 Mouse anti-Human, Brilliant Violet 480, Clone: MI15, BD

Description

• The MI15 monoclonal antibody specifically binds to CD138 (Syndecan-1), an 85-92 kDa single chain transmembrane protein, which is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations
• It is also expressed on pre-B cells, immature B cells, and plasma cells, but not on mature circulating B-lymphocytes
• Syndecan-1 is a member of the transmembrane heparan sulfate proteoglycans family
• It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial and neural cells
• CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III
• It is considered an extracellular matrix receptor that may serve as a co-receptor for fibroblast growth factor and related molecules
• Monoclonal antibody MI15 blocks the binding of clone B-B4 but not clone DL-101 (other anti-syndecan-1 antibodies) by flow cytometric analysis
• The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40nm) filter set
• BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510
• BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40nm) filter set
• BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510.  Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously.