CD3 Mouse anti-Human, Alexa Fluor 647, Clone: MEM-57, BD

Description

• The human CD3 complex consists of four transmembrane proteins (γ, δ, ε, ζ) that are associated withthe T cell antigen receptor (TCR) to form the CD3/TCR complex
• The CD3 complex associates witheither TCR αβ or TCR γδ heterodimersthat are expressed by some thymocytes, T cells or NKT cells
• The CD3 complex is required for the cell surface expression and signal-transducing functions of the TCR
• The CD3 complex is expressed by ñ60-85% thymocytes and ñ60-80% of normal human peripheral blood lymphocytes
• The MEM-57 monoclonal antibody reportedly recognizesthe human CD3 epsilon subunit (CD3e/CD3ε, also known as T3E or TCRE) of the CD3/TCR complex
• CD3e is a ñ20 kDa unglycosylated transmembrane protein that belongs to the Ig gene superfamily
• CD3e has an Ig-like extracellular domain (ECD) and an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain
• The MEM-57 antibody reportedly stimulates T cell proliferation and can induce cytotoxicity in some human T cell lines
• Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range
• The excitation and emission maxima are nearly identical to those of APC
• However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications
• This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy
• Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.