IRF-3 Mouse anti-Human, Alexa Fluor 647, Clone: SL-12.1 (also known as SL-12), BD

Description

• The SL-12.1 monoclonal antibody specifically recognizes human Interferon regulatory factor 3 (IRF-3)
• Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism
• One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNαand IFNβ)
• These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules
• These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs)
• IRFs are a family of transcription factors that possess a broad range of activities
• IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters
• The IRF3 /gene expresses a 50 kDa protein which is constitutively expressed in all tissues
• The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA
• Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range
• The excitation and emission maxima are nearly identical to those of APC
• However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications
• This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy
• Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.