CD127 Rat anti-Mouse, Brilliant Violet 421, Clone: A7R34, BD

Description

• The A7R34 monoclonal antibody specifically binds to mouse CD127 which is also known as the Inter )
• CD127 associates with CD132 ( ) to form a high-affinity signaling IL-7R complex that mediates the biological functions of IL-7
• CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP
• CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies
• Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities
• CD127 is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, the earliest thymocyte progenitors, CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, naοve and memory T lymphocytes, + T cells, thymic NK cells, and bone marrow stromal cells
• CD127 is downregulated upon activation of naοve T cells and CD127 re-expression identifies effector cells that subsequently differentiate into memory T cells
• The A7R34 antibody blocks IL-7 binding to CD127
• This antibody has been used in both in vitro and in vivo studies to define the biological roles played by CD127
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.