Fyn Mouse, Unlabeled, Clone: 25, BD

Description

• Stimulation of the T cell antigen receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases
• The src-related p59[fyn] kinase is one such enzyme that associates with the TcR
• Ligation of the TcR activates the protein kinase activity of p59[fyn] invarious human T cells
• Fyn interacts with the CD3-ζ chains through its N-terminal region
• In turn, fyn binds other proteins through its SH2 and SH3 domains
• These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity
• A 72kDa fyn-related protein binds to the TcR/CD3 complex in certain T cells
• This protein, known as p72[fyn-R], is a variant of the fyn kinase and contains at least four novel Ser/Thr phosphorylation sites
• These sites may make p72[fyn-R] less susceptible to the action of phosphatases than p59[fyn]
• Like p59[fyn], p72[fyn-R] is constitutively associated with its receptor
• The exact relationship between these two proteins is not yet clear
• However, p72[fyn-R] immunoprecipitates with various antisera raised to either the N- or C-terminus of fyn
• Similar to src and yes, fyn is highly expressed in brain
• The localization of fyn in mouse brain and its interaction with myelin-associated glycoprotein (MAG) suggests that the fyn kinase has a role in the sensory network and in myelination during early stages of CNS formation
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting