PKCδ Mouse, Unlabeled, Clone: 14, BD

Description

• The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases
• At least eleven isozymes have been described
• These proteins are products of multiple genes and alternative splicing
• PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V)
• The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts withPKC activators Ca 2 + , phospholipid, diacylglycerol, or phorbol ester
• However, the novel PKC (nPKC) subfamily members (δ, ε, η and θ isoforms) and the atypical PKC (aPKC) subfamily members (ζ, ι, and λ isoforms) are Ca 2 + independent and lack the C2 domain
• The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters
• They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC
• The C-terminal region of PKC contains the catalytic domain
• PKCδ is involved in myeloid differentiation, as well as in the secretory response of antigen-stimulated rat basophilic RBL 2H3 cells
• Overexpression and subsequent stimulation of PKCδ leads to cell cycle arrest in CHO cells and complete growth inhibition of NIH 3T3 cells
• PKCδ is the most abundant isoform in hemopoietic cells and is highly expressed in many other organs and tissues
• This suggests that PKCδ may be one of the major PKC isozymes in mammalian cells
• This antibody is routinely tested by Western blot analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.