CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: UCHT1, BD Horizon™

Description

• The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
• CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
• Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
• CD3 plays a central role in signal transduction during antigen recognition
• The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).