BDB740026

CD14 Rat anti-Mouse, Brilliant Violet 421, Clone: rmC5-3, BD Optibuild™

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB740026-Each-of-1 In Stock ₹ 34,224.00

BDB740026 - Each of 1

₹ 34,224.00

In Stock

Quantity

1

Base Price: ₹ 34,224.00

GST (18%): ₹ 6,160.32

Total Price: ₹ 40,384.32

Antigen

CD14

Classification

Monoclonal

Concentration

0.2mg/mL

Description

Cd14; CD14 antigen; Myeloid cell-specific leucine-rich glycoprotein

Host Species

Rat

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG1 κ

Applications

Flow Cytometry

Clone

rmC5-3

Conjugate

Brilliant Violet 421

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

Recombinant Mouse CD14

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Related Products

Img

BD Biosciences

BDB740692

--

Img

BD Biosciences

BDB740125

--

Img

BD Biosciences

BDB740357

--

Img

BD Biosciences

BDB740486

--

Img

BD Biosciences

BDB740241

--

Img

BD Biosciences

BDB740773

--

Img

BD Biosciences

BDB740163

--

Img

BD Biosciences

BDB740286

--

Description

  • The rmC5-3 monoclonal antibody specifically binds to residues 308-322 of the hydrophilic region of mouse CD14
  • CD14 is a 53-55 kDa glycophosphatidylinositol (GPI)-linked glycoprotein belonging to the leucine-rich glycoprotein repeat superfamily of cell-surface proteins
  • It is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein)
  • CD14 serves as a receptor for LPS that can play a role in the cellular production of proinflammatory cytokines such as IL-1 and TNF
  • CD14 can be involved in the development of endotoxic shock and LPS-stimulated bone resorption, and promotes, possibly indirectly, bacterial dissemination
  • Flow cytometric analysis demonstrates that rmC5-3 antibody stains J774A.1 (mouse macrophage line), WEHI-265.1 (mouse monocytic line), peritoneal resident macrophages, Kupffer cells, and cultured bone marrow-derived macrophages and dendritic cells, but not unstimulated splenic macrophages, dendritic cells, neutrophils, or blood monocytes
  • This staining pattern is similar to that of the alternate anti-mouse CD14 mAb 4C1/CD14, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils
  • Therefore, data suggests that CD14 expression by leukocyte populations may differ in mice and humans
  • Peritoneal cells from naive mice, 3-day thioglycollate-elicited peritoneal exudate, as well as 4-hour LPS-activated peritoneal cells, contain a population of Mac-1 (CD11b)-high cells which double-stain with rmC5-3 antibody
  • Levels of CD14 expression on Kupffer cells and bone marrow-derived macrophages and dendritic cells of LPS-sensitive mice are increased by in vivo and in vitro LPS treatments, an effect which may be mediated by TNF
  • Preliminary evidence suggests that CD14 may be up-regulated on mouse blood neutrophils
  • In agreement with the observations that CD14 is shed from activated human and mouse monocytes, rmC5-3 mAb detects soluble CD14 in the serum of LPS-treated mice in a time-dependent manner
  • The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
  • BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Compare Similar Items

Show Difference

Img

BD Biosciences

BDB740026

--


Antigen:
CD14

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Cd14; CD14 antigen; Myeloid cell-specific leucine-rich glycoprotein

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
rmC5-3

Conjugate:
Brilliant Violet 421

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Recombinant Mouse CD14

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

BD Biosciences

BDB740027

--


Antigen:
CD62E

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Sele; E-selectin; Elam; ELAM-1; LECAM2; LYAM2

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
10E9.6

Conjugate:
Brilliant Violet 421

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Mouse brain capillary endothelioma bEnd.3 (TNFα-stimulated)

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

BD Biosciences

BDB740028

--


Antigen:
CD27

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Tnfrsf7; Tumor necrosis factor receptor superfamily member 7; Tp55; S152

Host Species:
Hamster

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
LG.3A10

Conjugate:
Brilliant Violet 421

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

BD Biosciences

BDB740029

--


Antigen:
CD35

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
CR1, CD21b

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
8C12

Conjugate:
Brilliant Violet 421

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Purified mouse CR1

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.