CD90/CD90.1 Mouse anti-Mouse, Brilliant Violet 510, Clone: HIS51, BD Optibuild™

Description

• The HIS51 monoclonal antibody specifically binds to the rat Thy-1 antigen (CD90) expressed by hematopoietic stem cells, early myeloid and erythroid cells, immature B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T cells), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and bone marrow-derived dendritic cells
• Rat dendritic epidermal T cells (DEC) are Thy-1-negative
• CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction
• In addition, there is evidence in the mouse that CD90 mediates adhesion of thymocytes to thymic stroma
• HIS51 antibody cross-reacts with the mouse Thy-1.1 alloantigen of the AKR/J and PL strains, but not Thy-1.2 found on most strains
• In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells, and neurons, but not B lymphocytes
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.