CD54 Mouse anti-Human, Brilliant Violet 605, Clone: HA58, BD Optibuild™

Description

• The HA58 monoclonal antibody specifically binds to CD54 which is also known as ICAM-1 (intracellular adhesion molecule-1)
• CD54 is an 85-110 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin supergene family
• A soluble form of CD54 can also be found in biological fluids
• CD54 is expressed on endothelial cells and both resting (weak) and activated (moderate) lymphocytes and monocytes
• CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18)
• The CD54 adhesion molecule plays roles in inflammatory and immune responses and neoplasia
• This antibody (NA/LE format) blocks the mixed-lymphocyte reaction (MLR) and the purified format is suitable for staining acetone-fixed, frozen tissue sections
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.