CD25 Rat anti-Mouse, Brilliant Violet 650, Clone: 3C7, BD Optibuild™

Description

• The 3C7 monoclo nal antibody specifically binds to CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested
• IL-2Rα by itself is not a signaling receptor
• However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2
• Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα
• CD25 is transiently expressed at a low level during normal B-cell development in th e bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells
• It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus
• Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance
• It has also been reported that dendritic cells express CD25, recognized by mAb 7D4, another CD25-specific antibody
• The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by other CD25-specific mAbs, 7D4 and PC61
• 3C7 blocks the binding of IL-2 to CD25
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.