CD28 Hamster anti-Mouse, Brilliant Violet 650, Clone: 37.51, BD Optibuild™

Description

• The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells
• The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation
• CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells
• It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes
• CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2)
• The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction
• There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.