BDB740490

CD45.2 Mouse anti-Mouse, Brilliant Violet 650, Clone: 104, BD Optibuild™

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB740490-Each-of-1 In Stock ₹ 34,052.88

BDB740490 - Each of 1

₹ 34,052.88

In Stock

Quantity

1

Base Price: ₹ 34,052.88

GST (18%): ₹ 6,129.518

Total Price: ₹ 40,182.398

Antigen

CD45.2

Classification

Monoclonal

Concentration

0.2mg/mL

Description

Ly-5.2; T200; LCA; Leukocyte common antigen; Ptprc

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG2a κ

Applications

Flow Cytometry

Clone

104

Conjugate

Brilliant Violet 650

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

B10.S mouse thymocytes and splenocytes

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The 104 monoclonal antibody recognizes the CD45 (Leukocyte Common Antigen) present on all leucocytes of most mouse strains (eg, A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129)
  • This alloantigen was originally named Ly-5.1, and this was the designation at the time that the antibody was characterized
  • The designation was later changed from Ly-5.1 to Ly-5.2 to conform with the convention that the .2 alloantigen designations be assigned to the C57BL/6 strain
  • mAb 104 has been reported not to react with leucocytes of the mouse strains expressing the CD45.1 alloantigen (eg, RIII, SJL/J, STS/A, and DA)
  • CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation
  • The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific
  • The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction
  • The 104 antibody has been reported to inhibit some responses of B cells, from mice expressing the CD45.2 alloantigen, to certain antigens and LPS
  • In addition, reduction of serum IgG levels and amelioration of autoimmune renal pathology were reported in mAb 104-treated systemic lupus erythematosus-prone mice
  • The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
  • BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination.

Compare Similar Items

Show Difference

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BD Biosciences

BDB740490

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Antigen:
CD45.2

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Ly-5.2; T200; LCA; Leukocyte common antigen; Ptprc

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
104

Conjugate:
Brilliant Violet 650

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
B10.S mouse thymocytes and splenocytes

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
CD27

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Tnfrsf7; Tumor necrosis factor receptor superfamily member 7; Tp55; S152

Host Species:
Hamster

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
LG.3A10

Conjugate:
Brilliant Violet 650

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
CD40

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Bp50; Tnfrsf5; TNR5; TRAP; CD40L receptor; GP39; HIGM1; IMD3; T-BAM

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
3/23

Conjugate:
Brilliant Violet 650

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Mouse CD40 Recombinant Protein

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
LPAM-1

Classification:
Monoclonal

Concentration:
0.2mg/mL

Description:
Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
DATK32

Conjugate:
Brilliant Violet 650

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
AKR/Cum mouse T lymphoma line TK1

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.