CD140a Rat anti-Mouse, Brilliant Violet 650, Clone: APA5, BD Optibuild™

Description

• The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR- α )
• CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult
• CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells
• CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain
• Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms
• Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization
• The APA5 antibody has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.