CD16 Mouse anti-Human, Brilliant Violet 650, Clone: B73.1, BD Optibuild™

Description

• The B73.1 monoclonal antibody specifically binds to CD16, the 50-70 kDa low affinity receptor for IgG, IgG Fc receptor III (Fc γRIII)
• CD16 is expressed on NK cells, neutrophils, and on a subset of T cells from certain individuals
• The B73.1 antibody binds to CD16-positive neutrophils with lower intensity when compared with some other CD16-specific antibodies
• A variable number of CD16-positive lymphocytes coexpress either the CD57 antigen or low-density CD8 antigen or both
• The B73.1 antibody can block Fc receptor functions mediated by CD16
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.