CD29 Hamster anti-Mouse, Brilliant Violet 711, Clone: HM β1-1, BD Optibuild™

Description

• The HM β 1-1 monoclonal antibody specifically binds to the 130-kDa integrin β 1 chain (CD29)
• CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin- α chains
• With α 1 through α 6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with α v (CD51), it forms α α 8, and α 9 chains in non-lymphoid tissues
• As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes
• This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes
• Source of the immunogen was purified mouse VLA-4 ( α 4β 1, CD49d/CD29)
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.