CD122 Rat anti-Mouse, Brilliant Violet 786, Clone: 5H4, BD Optibuild™

Description

• The 5H4 antibody monoclonal antibody specifically binds to mouse CD122
• CD122 is a 70-85 kDa type I transmembrane glycoprotein of the hematopoietin receptor superfamily
• CD122 is also known as IL-2 Receptor beta chain (IL-2R β ) or IL-15 Receptor beta chain (IL-15R β ) as it helps form signaling receptor complexes for Interleukin-2 (IL-2) or IL-15, respectively
• CD122 can combine with either CD132 (γc) alone or CD132 plus CD25 (IL-2Rα) to form inte rmediate or high-affinity IL-2 Receptor complexes, respectively
• The IL-2R β chain is constitutively expressed on NK cells and at lower levels on some thymocytes, T cells, B cells, monocytes, and macrophages
• IL-2R β chain expression is up regulated by IL-2
• The immunogen used to generate this hybridoma was rat myel oma cells transfected with a truncated IL-2R β cDNA
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).