CD102 Rat anti-Mouse, BB700, Clone: 3C4(mIC2/4), BD Optibuild™

Description

• The 3C4 (mIC2/4) monoclonal antibody specifically binds to the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1
• CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls
• It is also expressed on a variety of leukocyte cell lines
• CD102 does not appear to be involved in the development of hematopoietic cells
• In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen
• The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.