CD38 Rat anti-Mouse, BB700, Clone: 90/CD38 (also known as Ab90), BD Optibuild™

Description

• The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes
• In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells
• CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages
• Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells
• The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation
• CD31, both human and mouse, is reported to be a ligand for human CD38
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.