CD49a Hamster anti-Mouse, BB700, Clone: Ha31/8, BD Optibuild™

Description

• Description The Ha31/8 monoclonal antibody specifically binds to the 180-kDa integrin á1 chain (CD49a), which is a transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the α1β1 (complex known as VLA-1)
• VLA-1 has been reported to be expressed on activated T cells, monocytes, smooth muscle cells, and endothelial cells
• It is a receptor for collagen and laminin
• The Ha31/8 monoclonal antibody is specific for both rat and mouse CD49a
• It has been reported that Ha31/8 antibody can block VLA-1-mediated binding of rat cells to collagen
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.