CD22 Mouse anti-Human, BB700, Clone: HIB22, BD Optibuild™

Description

• The HIB22 monoclonal antibody specifically binds to CD22
• CD22 is a 130-140 kDa glycosylated type I integral membrane protein present on the surface of mature B cells
• CD22 is expressed in the cytoplasm of virtually all B cells except plasma cells
• CD45RO antigen on T cells and CD75 antigen on B cells have been identified as ligands for CD22
• CD22 has been reported to participate in B-cell activation and also as an adhesion molecule
• Although the immunobiology of this antigen has not been fully elucidated, reports indicate that ligation of CD22 induces constitutive internalization of the molecule followed by complete degradation
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.