Notch2 Mouse anti-Human, Brilliant Violet 650, Clone: MHN2-25, BD Optibuild™

Description

• The MHN2-25 monoclonal antibody specifically binds to an extracellular domain of human Notch2
• Notch2 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4
• Notch2 is cleaved in the Golgi and presents as a cell surface heterodimeric receptor
• The Notch2 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2, Delta1 and Delta4
• Upon ligand binding, Notch2 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD
• NICD translocates to the nucleus where it forms a transcriptional activator complex with various transcriptional factors
• These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of multiple organ systems such as the hematopoietic, immune, cardiovascular, hepatic and renal systems
• Notch2 is expressed by cells of the lung and brain, as well as cells of the B lineage, thymocytes, and T cells
• Abnormalities in Notch2 expression may play a role in the development of B cell lymphomas
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.