CD132 Mouse anti-Human, Brilliant Violet 650, Clone: AG184, BD Optibuild™

Description

• The AG184 monoclonal antibody specifically binds to the 65-70 kDa commonγsubunit (γc) that is shared by the IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 receptor complexes
• Theγc receptor is a type 1 transmembrane glycoprotein that is constitutively expressed by most peripheral T and B lymphocytes, NK cells, monocytes and granulocytes
• The cytoplasmic domain of theγc chain plays an important role in cytokine-mediated signal transduction
• By immunofluorescent staining and flow cytometric analyses, the AG184 antibody has been shown to specifically recognize humanγc expressed by cell lines including humanγc gene-transfected cell lines which are known to express the humanγc chain
• The AG184 antibody can bind theγc chain in the receptors complexed with IL-2, IL-4, or IL-7, indicating that the antibody recognizes an epitope which is distinct from the cytokine binding site of theγc chain
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.