CD28 Mouse Anti-Rat, Brilliant Violet 711, Clone: JJ319, BD Optibuild™

Description

• The JJ319 monoclonal antibody specifically recognizes CD28, which is expressed on virtually all T lymphocytes bearingαβT-cell receptors (TCR), on mostγδTCR-bearing T cells, and on a subset of NK cells
• In the thymus, CD28 expression is developmentally regulated during the maturation ofαβTCR-bearing T cells
• CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2)
• Soluble JJ319 mAb costimulates the proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-αβ-TCR mAb R73 (Cat
• no
• 554910)
• The alternate anti-rat CD28 mAb JJ316 (Cat
• No
• 554992) is capable of directly stimulating T cells in vitro and in vivo
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.