CD59 Mouse Anti-Rat, BUV395, Clone: TH9, BD Optibuild™

Description

• The TH9 antibody monoclonal antibody specifically binds to CD59, a 21 kDa glycosyl-phosphatidyl inositol-anchored cell-surface glycoprotein of the Ly-6 superfamily
• CD59 is expressed by many types of non-hematopoietic cells
• In the rat hematopoietic system, CD59 has been detected on erythrocytes, monocytes, and some lymphocytes, but not on platelets
• Soluble CD59 is found in body fluids and urine
• CD59 is a complement regulatory protein that acts late in the complement cascade to prevent formation of the membrane attack complex (MAC)
• Therefore, CD59 is one of several proteins whose function is to protect host tissue from complement attack
• Rat CD59 binds rat and human complement components and inhibits cytolysis mediated by complement from multiple species
• CD59 has also been suggested to be a ligand for CD2 and to participate in T-cell costimulation
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.