CD4 v4 Mouse anti-Human, Brilliant Violet 711, Clone: L120, BD Optibuild™

Description

• The L120 monoclonal antibody specifically recognizes CD4
• CD4 is an ñ59 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily
• CD4 is expressed on helper-inducer subset of T lymphocytes and monocytes
• The CD4 antigen is involved in the Major Histocompatibility Complex (MHC) class II-restricted recognition of foreign antigens and has been identified as a receptor for HIV
• The L120 antibody recognizes amino acids 306-370 of the antigen and does not block the binding of the gp120 of HIV, but inhibits the proliferation of CD4 T lymphocytes to MHC class II antigens
• The CD4 antigen is present on most thymocytes, approximately 40% of peripheral blood lymphocytes, and at lower density on monocytes
• Peripheral blood CD4+ T lymphocytes are lost over time during HIV infection, and this loss is correlated with disease progression
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.