CD66c Mouse anti-Human, Brilliant Violet 421, Clone: B6.2/CD66, BD Optibuild™

Description

• The B6.2 monoclonal antibody reacts with a glycosylphosphatidylinositol-anchored glycoprotein present on granulocytes
• Antibody B6.2 was studied as recognizing CD66c in the VI Human Leukocyte Differentiation Antigen workshop
• CD66 antigens also known as the carcinoembryonic antigen (CEA) family of molecules, are closely related to the immunoglobulin super family of glycoproteins
• Studies on CD66 molecules suggest a potential adhesion function in vivo
• These molecules exhibit both homophilic and heterophilic adhesion
• CEA family members may be involved in transmembrane signalling and activation of neutrophils
• This clone has been found to be N-terminal domain reactive, reacted preferentially with the native protein and were conformationally dependent The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.