ASGPR 1 Mouse anti, Brilliant Violet 650, Clone: 8D7, BD Optibuild™

Description

• The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1)
• ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells
• It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane
• ASGPR 1 plays a role in serum glycoprotein homeostasis
• It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation
• The receptor may also promote hepatic infection by the binding and uptake of various viruses
• The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts
• Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3)
• The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1
• Clone 8D7 cross-reacts with human ASGPR 1
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.