CD58 Mouse anti-Human, Brilliant Violet 711, Clone: 1C3 (also known as AICD58.6), BD Optibuild™

Description

• The 1C3 (AICD58.6) monoclonal antibody specifically binds to CD58
• CD58 is a a 60-70 kDa glycoprotein member of the immunoglobulin superfamily
• CD58, also referred to as the lymphocyte function-associated antigen-3 (LFA-3), has a wide tissue distribution, being expressed on both hematopoietic and non-hematopoietic cells, including endothelial cells and fibroblasts
• There are two isoforms of CD58: a glycosylphosphatidylinositol (GPI)-linked form and a transmembrane form
• Both isoforms may be expressed on the same cell type
• Erythrocytes, however, only express the GPI-linked isoform
• CD58 interacts with CD2 during cell adhesion
• This binding can enhance antigen-specific T-cell activation
• This interaction can also play a role in cell-mediated cytotoxicity
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.