CD195 Mouse anti-Human, BUV395, Clone: 3A9, BD Optibuild™

Description

• The 3A9 monoclonal antibody recognizes CD195, which is also known as the chemokine receptor, CCR5, a seven transmembrane-spanning G protein-associated molecule
• The 3A9 antibody also reportedly cross-reacts with human CCR8
• Results of epitope mappingand sequence comparison between CCR5 and CCR8 reveals that the first three amino acid residues for these two receptors are identical: MDY (Met-Asp-Tyr)
• CCR5 belongs to the β-chemokine receptor family
• It is expressed on subsets of T lymphocytes, NK cells, monocytes, macrophages, and dendritic cells
• CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation
• It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1) α and β
• Additionally, CCR5 has beenfound to be a co-receptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission
• Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection
• CCR5 has been clustered as CD195 in the VIIth HLDA workshop
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.