CD140b Mouse anti-Human, BUV395, Clone: 28D4, BD Optibuild™

Description

• Reacts with platelet derived growth factor (PDGF) receptorβ, a 170-190 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes
• PDGF receptors,αandβ, are single glycoproteins with intracellular tyrosine kinase domains
• They are structurally similar to M-CSF receptor and CD117 (c-kit)
• Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells
• PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils
• Functional PDGF is secreted as a disulfide-linked dimer of A and B chains (PDGF-AA, PDGF-BB or PDGF-AB)
• Binding of divalent PDGF induces receptor dimerization with three possible forms:αα,αβ,ββ
• The PDGF receptorα-subunit binds both PDGF A and B chains, whereas the receptorβ-subunit binds only PDGF B chain
• Both receptor subunits can stimulate mitogenic responses, only theβsubunit can induce chemotaxis
• 28D4 is specific for PDGFRβand does not cross-react with PDGFRα
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.